Journal: Experimental and Therapeutic Medicine
Article Title: Silencing of kallikrein-related peptidase 6 attenuates the proliferation, migration, and invasion of gastric cancer cells through inhibition of epithelial-mesenchymal transition
doi: 10.3892/etm.2021.10202
Figure Lengend Snippet: KLK6 is highly expressed in HGC-27 cells. Relative (A) protein and (B) mRNA expression levels of KLK6 in HGC-27, AGS and SNU-1 cells. Relative (C) protein and (D) mRNA expression levels of KLK6 in HGC-27 cells after plasmid transfection for 24 h. Relative (E) protein and (F) mRNA expression of KLK6 in HGC-27 cells after overexpression plasmid transfection for 24 h. Data are presented as the mean ± SD (n=3). KLK6, kallikrein-related peptidase 6; Con, untreated control; shRNA, short hairpin RNA; NC, non-coding control; OV, overexpression plasmid; n.s., not significant. *P<0.05.
Article Snippet: Cell transfection pSICOR interference vectors and pCDH-CMV- MCS-EF1-CopGFP-T2A-Puro overexpression vectors were supplied by Addgene, Inc. KLK6 mRNA was amplified via PCR (forward, 5'-GCTCTAGATGAAGAAGCTGATGGTG-3'; reverse, 5'-CGGGATCCTCACTTGGCCTGAATGGT-3') and inserted into the pCDH vector to produce pCDH-KLK6 overexpression (OV) plasmids.
Techniques: Expressing, Plasmid Preparation, Transfection, Over Expression, Control, shRNA